ß d glucan  (MedChemExpress)

Journal: Advanced Science

Article Title: Trained Immunity Exacerbates Inflammatory Arthritis Progression via Promoting Synovial Fibroblast Ferroptotic Resistance

doi: 10.1002/advs.202504245

Figure Lengend Snippet: Trained immunity affects inflammatory arthritis development in an established CIA rat model. A) Schematic experimental induction of trained immunity in the CIA rat model, B) Representative images of the hind paw of rats with or without β‐glucan training on day 35 ( n = 1), C) Hind paw thickness was calibrated from the second immunisation onwards ( n = 6), D) Arthritis scores were monitored according to A ( n = 6), E) Hematoxylin and eosin (H&E) images of representative knee and ankle in rats at day 35, scale bars, 200 µm., F,G) Levels of IL‐β and TNF‐a in the serum (G) or joint effusion (H) of rats ( n = 6)., H) Representative immunofluorescence assays and the proportion of IL‐β + VCAM + or IL‐β + /F4/80 + cells in the joints of rats, representative sections per rat, scale bars, 50 µm., In C, D, F, G, data are presented as mean ± SD, C & D, * p <0.05 by a Two‐Way ANOVA; F, G, * p <0.05 by a One‐way ANOVA. See also in Figure (Supporting Information).

Article Snippet: For Figure , rats were trained with β‐glucan (1.0 mg rat −1 , Invivogen, #tlrl‐wgp) in 100.0 μL PBS by intravenous route on day 0, and then injected subcutaneously with 200.0 μL emulsion (equivalent amounts of incomplete Freund's adjuvant (Chondrex, Redmond, USA) and bovine type II collagen (Chondrex, Redmond, USA)) (0.2 μg rat −1 ) unilaterally at the base of the tail on day 7.

Techniques: Immunofluorescence

Journal: Advanced Science

Article Title: Trained Immunity Exacerbates Inflammatory Arthritis Progression via Promoting Synovial Fibroblast Ferroptotic Resistance

doi: 10.1002/advs.202504245

Figure Lengend Snippet: CAI‐induced maladaptive inflammation enhances macrophages training, contributing to severity of arthritis, A) CIA rat model was initiated on day 0, followed by the induction of β‐glucan‐mediated trained immunity on day 14, B) Arthritis index and paw thickness were scored. * Compared with CIA ( ** p <0.01, ANOVA followed by multiple comparisons) ( n =5), C) The levels of IL‐β in the serum of rats ( n = 5), D) Representative photographs of morphology in different groups ( n =3), E) H&E staining of representative joints in different groups, scale bars, 200 µm, F) The level of IL‐β and IL‐10 in the joint effusion of rats ( n = 5), G) Immunofluorescence assays of IL‐β + VCAM + in the joints of rats, scale bars, 50 µm, H) The pathological progression of rheumatoid arthritis aggravated by trained immunity, In B, C, F, data are presented as mean ± SD, B, ** p <0.01 by a Two‐Way ANOVA, C, F, * p <0.05 by a One‐way ANOVA. See also in Figures and (Supporting Information).

Article Snippet: For Figure , rats were trained with β‐glucan (1.0 mg rat −1 , Invivogen, #tlrl‐wgp) in 100.0 μL PBS by intravenous route on day 0, and then injected subcutaneously with 200.0 μL emulsion (equivalent amounts of incomplete Freund's adjuvant (Chondrex, Redmond, USA) and bovine type II collagen (Chondrex, Redmond, USA)) (0.2 μg rat −1 ) unilaterally at the base of the tail on day 7.

Techniques: Staining, Immunofluorescence